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expression cloning : ウィキペディア英語版
expression cloning

Expression cloning is a technique in DNA cloning that uses expression vectors to generate a library of clones, with each clone expressing one protein. This ''expression library'' is then screened for the property of interest and clones of interest recovered for further analysis. An example would be using an expression library to isolate genes that could confer antibiotic resistance.
==Expression vectors==
Expression vectors are a specialized type of cloning vector in which the transcriptional and translational signals needed for the regulation of the gene of interest are included in the cloning vector. The transcriptional and translational signals may be synthetically created to make the expression of the gene of interest easier to regulate.

抄文引用元・出典: フリー百科事典『 ウィキペディア(Wikipedia)
ウィキペディアで「expression cloning」の詳細全文を読む



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